Part:BBa_K4774012:Design

CBD-SsrA+Npu-eGFP-Npu 2-CBD2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1363
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1363
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1363
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1363
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The composite part combines Enhanced Green Fluorescent Protein (eGFP, BBa_K4401002), Npu intein (BBa_K4774005 and BBa_K4774007), SsrA tag (BBa_K4774006) CBD (BBa_K4774002) and CBD2 (BBa_J70627).

Source

Genebank